切向流過濾
Tangential Flow Filtration，TFF
切向流過濾（Tangential Flow Filtration，TFF）是目前應用非常廣泛的一種膜分離方法。樣品在壓力驅(qū)動下，內(nèi)容物根據(jù)分子尺寸不同實現(xiàn)膜過濾分離，達到去除熱原、置換溶液環(huán)境、純化和濃縮等目的。
Tangential Flow Filtration (TFF) is a widely used membrane separation method. Driven by pressure, the contents of the sample can be separated by membrane filtration according to different molecular sizes, so as to remove pyrogen, replace solution environment, purify and concentrate.
切向流過濾的膜部件通常是膜包或者中空纖維柱，二者均有多種型號，以滿足不同的使用需求。膜材種類和孔徑大小、溶液循環(huán)流速、膜兩側(cè)壓力、樣品溫度、工藝時間等參數(shù)，是切向流過濾非常重要的參數(shù)，它們決定了最終的工藝效果，以及大規(guī)模生產(chǎn)的可行性及具體方案。實驗室規(guī)模的切向流過濾設備致力于獲得可行的工藝方案和可靠的工藝參數(shù)，為大規(guī)模試驗和生產(chǎn)確定適合的工藝參數(shù)，提供設備或系統(tǒng)選型的有效依據(jù)。
The membrane components of tangential flow filtration are usually membrane package or hollow fiber column, both of which have a variety of models to meet different use needs. Membrane material type and pore size, solution circulation flow rate, pressure on both sides of the membrane, sample temperature, process time and other parameters are very important parameters for tangential flow filtration. They determine the final process effect, as well as the feasibility and specific scheme of large-scale production. The laboratory scale tangential flow filtration equipment is committed to obtaining feasible process schemes and reliable process parameters, determining appropriate process parameters for large-scale test and production, and providing an effective basis for equipment or system selection.
包裹小分子藥物的脂質(zhì)體和包裹核酸藥物的脂質(zhì)納米顆粒是當下非常熱門的兩類藥物載體，他們在研發(fā)和生產(chǎn)中均需使用切向流過濾處理樣品。
Liposomes coated with small molecule drugs and lipid nanoparticles coated with nucleic acid drugs are two very popular drug carriers. They need to use tangential flow filtration to treat samples in R & D and production.
圖1：超濾系統(tǒng)流程圖
Figure 1: flow chart of ultrafiltration system
從濾膜的截留孔徑進行分類，可以將過濾分為澄清過濾、微濾、超濾、納濾和反滲透等。將不同孔徑的濾膜采用切向流的操作方式進行過濾，即可稱之為切向流微濾和切向流納濾等。
The filtration can be divided into clarification filtration, microfiltration, ultrafiltration, nanofiltration and reverse osmosis. Tangential flow microfiltration and tangential flow nanofiltration can be referred to as tangential flow microfiltration and tangential flow nanofiltration.
圖2：濾膜孔徑分類
Figure 2: filter membrane pore size classification
技術(shù)參數(shù)
Technical Parameter
|
型號 |
LDS-L2 |
|
最小樣品體積 |
30 mL |
|
壓力范圍 |
-1 - 5 bar |
|
流速范圍 |
1 - 2200 mL/min |
|
重量范圍 |
0 – 4100 g |
|
可顯示數(shù)據(jù) |
Pf（進料壓力），Pr（回流壓力），Pp（透過壓力），TMP（跨膜壓），△P（壓降）等 |
|
操作方式 |
自動（LDS-L2） |
|
適用容器 |
玻璃容器，不銹鋼容器等 |
|
適用膜材 |
PES/纖維素膜包，中空纖維柱 |
|
膜包規(guī)格 |
5 – 1000 kD；0.01 – 0.1 m2 |
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